HPTLC High Performance Thin Layer Chromatography is a modernized version
of TLC technique. The underlying principle remains the same i.e. planar
chromatography.
HPTLC gives better separation and resolution of bands compared to TLC
technique. Also, quantification aspect is added in HPTLC which an analyst won’t
achieve using conventional TLC.
HPTLC has a wide area of applications ranging from herbal, food,
pharmaceuticals to forensics. Let us have a look at what kind of analyses can
be done using CAMAG HPTLC.
Application of herbals involve HPTLC fingerprinting where there is no
requirement of marker/standard. Fingerprint is the individual chromatographic track
representing all the constituents in a plant sample. This allows
us to differentiate at species to species level. Class of compounds is a
phytochemical test confirming the presence of functional groups such as
phenols, flavonoids, alkaloids and antioxidants etc. This type of analysis is
qualitative.
Applications in the field of
pharmaceuticals involve various types of analysis such quantification,
validation studies, limit tests, impurity profiling, related substance and
content uniformity test (CUT).
Forensic application involves poison investigations. Various food and
FDA laboratories in India use HPTLC for detection of adulteration, aflatoxins
and spurious compounds.
Other types of analyses which can be done using HPTLC involve stability studies and forced degradation studies.
Effect directed analysis which involves DPPH assay (antioxidant assay) and bioautography.
Hyphenation is one useful tool which combines two unique chromatographic
techniques to give best output. Double confirmation using HPTLC-MS is possible.
This facilitates identification of unknown compounds without any tedious sample
clean up.
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