HPTLC association has developed a method for Cyperus rotundus rhizome. This method also discriminates C. rotundus from other related species. This method is adopted in the International HPTLC Atlas for the identification of herbal drugs and in the British Pharmacopoeia, 2018. Standards isoeugenol and isoeugenyl acetate were used.
In TLC/HPTLC, basically, two phases are important i.e. stationary phase and mobile phase. The gas phase also plays an important role if we are giving saturation.
The monograph is a scientific document covering plant research from different aspects. It includes information about the particular plant like scientific name, other common names, morphological and anatomical characteristics, chemical constituents, TLC/HPTLC/HPLC/GC identity test, adulterants/substitutes, pharmacology, major therapeutic claims, safety aspects, and dose.
HPTLC is the only chromatographic technique that allows us to see/record results in the form of pictures. Visual detection and image recording are one of the best features of the HPTLC system. For image documentation, a suitable image acquisition system can be used e.g. CAMAG smartDigi or CAMAG visualizer.