{"id":601,"date":"2022-09-01T12:59:23","date_gmt":"2022-09-01T12:59:23","guid":{"rendered":"https:\/\/anchrom.in\/blogs\/?p=601"},"modified":"2022-09-01T13:00:17","modified_gmt":"2022-09-01T13:00:17","slug":"how-is-chromatography-used-to-study-plant-pigments","status":"publish","type":"post","link":"https:\/\/anchrom.in\/blogs\/how-is-chromatography-used-to-study-plant-pigments\/","title":{"rendered":"Chromatography for Plant Pigments"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>How chromatography is used to study plant pigments<\/strong>?<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Words \u2018Plant pigments\u2019 and \u2018chromatography\u2019 reminds us of the beginning of chromatographic science. A Russian Botanist \u2018Mikhail Tsvet\u2019 invented chromatography in 19<sup>th<\/sup> century during his research on separation of plant pigments. Tsvet had managed to separate a mixture of plant pigments, including chlorophyll, on a column packed with finely ground calcium carbonate, using petroleum ether as the mobile phase.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Natural pigments such as chlorophylls, chlorophyll derivatives, xanthophylls, carotenoids, anthocyanins, betacyanin\u2019s are abundant in plants or plant derived products. Plant parts such as coloured flowers and fruits are because of carotenoids, flavonoids, anthocyanins or betalains. There are two main groups of betalains, the red-violet betacyanins and the yellow betaxanthins. Anthocyanins can be extracted by using solvent like methanol and often acid is added while doing extraction as most of the anthocyanins are pH sensitive.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Normal phase or reversed phase plates are used for separation of plant pigments depending on the nature of pigments intended to be separated. The flavonoid pigments are one of the numerous and most widespread groups of natural products. The flavonoid class anthocyanins is the source of orange to blue colors in petals, fruits, leaves and roots. Other flavonoid contributes to yellow flower color either by cooccurring with carotenoid or by replacing them in about 15% of all plant species.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Until now various anthocyanin standards have been identified in different plant species e.g. pelargonidin, cyanidin, peonidin, delphinidin, petunidin and malvidin etc. Different solvent systems are used depending on type of stationary phase to be used. For normal phase plates solvent system like n butanol: acetic acid: water 40:10:20 v\/v\/v, ethyl acetate: isopropanol: water 65:25:10 v\/v\/v, Ethyl acetate: toluene: water: formic acid 10:3:0.8:1.1 v\/v\/v\/v. For reverse phase solvent systems like methanol: water: formic acid 40:60:2 v\/v\/v can be used.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Let\u2019s take one example to understand how HPTLC can be used for separation of plant pigments (In this case flavonoids) for the separation and to see differentiation between yellow and red Butea flowers:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Comparative HPTLC fingerprint of <em>Butea monosperma<\/em> (Yellow and Red flowers):<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-2.png\" alt=\"\" class=\"wp-image-602\" width=\"449\" height=\"397\" srcset=\"https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-2.png 380w, https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-2-300x265.png 300w\" sizes=\"(max-width: 449px) 100vw, 449px\" \/><figcaption>Red Butea flower<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"464\" height=\"341\" src=\"https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-3.png\" alt=\"\" class=\"wp-image-603\" srcset=\"https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-3.png 464w, https:\/\/anchrom.in\/blogs\/wp-content\/uploads\/2022\/08\/image-3-300x220.png 300w\" sizes=\"(max-width: 464px) 100vw, 464px\" \/><figcaption>Yellow Butea flower<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">HPTLC method was developed for differentiation between <em>Butea monosperma<\/em> yellow flowers and <em>Butea monosperma<\/em> red flowers.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">After development plate was derivatized with Natural product and anisaldehyde sulphuric acid reagent and image was taken at 366nm. Yellow fluorescent bands were observed at Rf 0.23, 0.34 in Red butea flowers which differentiates red from yellow butea flowers.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Whereas, aqua green fluorescent bands were observed at Rf 0.17, 0.23, 0.34 and 0.43 in yellow butea flowers only. These bands can be used for differentiation of yellow and red Butea flower samples.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Reference:<\/strong> Practical Thin Layer Chromatography-A Multidisciplinary Approach, B. Fried &amp; J. Sherma, CRC Press, Taylor and Francis Group, 2017.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Anchrom Enterprises Pvt. Ltd is one of the leaders in&nbsp;HPTLC in food analysis.&nbsp;Please contact us at&nbsp;<\/em><\/strong><a href=\"mailto:lab@anchrom.in\"><strong><em>lab@anchrom.in<\/em><\/strong><\/a><strong><em>&nbsp;for HPTLC analysis of plant extracts, drugs, ingredients in cosmetics, and forensic science.<\/em><\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"># HPTLC analysis of plant extracts<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"># HPTLC applications in India<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"># HPTLC chromatography applications in India<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"># Camag HPTLC herbal drug analysis<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"># Chromatographic fingerprinting of herbal products<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n","protected":false},"excerpt":{"rendered":"<p>Words \u2018Plant pigments\u2019 and \u2018chromatography\u2019 reminds us of the beginning of chromatographic science. A Russian Botanist \u2018Mikhail Tsvet\u2019 invented chromatography in the 19 th century during his research on the separation of plant pigments.<\/p>\n","protected":false},"author":4,"featured_media":605,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[4],"tags":[13,12,11,35,34,10,70,69,36],"class_list":["post-601","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hptlc-applications","tag-anchrom","tag-camag","tag-chromatography","tag-fingerprinting","tag-herbal","tag-hptlc","tag-pigments","tag-plant","tag-tlc"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Chromatography for Plant Pigments | Anchrom<\/title>\n<meta name=\"description\" content=\"Here, we have discussed a few tips on how chromatography can be used to study plant pigments. For more details, call us on 9833830898.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/anchrom.in\/blogs\/how-is-chromatography-used-to-study-plant-pigments\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Chromatography for Plant Pigments | Anchrom\" \/>\n<meta property=\"og:description\" content=\"Here, we have discussed a few tips on how chromatography can be used to study plant pigments. 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