{"id":781,"date":"2022-12-28T11:13:39","date_gmt":"2022-12-28T11:13:39","guid":{"rendered":"https:\/\/anchrom.in\/blogs\/?p=781"},"modified":"2023-03-03T11:25:42","modified_gmt":"2023-03-03T11:25:42","slug":"how-is-hptlc-different-from-tlc","status":"publish","type":"post","link":"https:\/\/anchrom.in\/blogs\/how-is-hptlc-different-from-tlc\/","title":{"rendered":"How is HPTLC different from TLC?"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Thin Layer Chromatography is a separation technique which is used to separate mixtures of organic compounds. This technique is in practice since 1960 and is been used for the separation of a lot of different compounds.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Thin Layer Chromatography technique and its Limitations:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Thin Layer chromatography is a manual technique. Nowadays some automation is introduced but still, complete automation is not there. The step-like sample application is done with capillary spotting, spotting causes a lot of diffusion and results in poor separation.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Plate development is done in a beaker or round bottom chamber and after development results are seen in the UV cabinet. If required derivatization is done by manual spraying which causes a lot of dripping of the reagent on the TLC plate and sometimes affects the resolution of the separated analyte.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Quantification is not possible because of poor separation and uncontrolled sample application. Besides this, there are a lot of environmental factors like temperature, humidity etc. which also contribute to poor separation. It does not meet modern regulatory requirements.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Traditional TLC is not suitable for QC because of several reasons such as noncompliance with GLP, non-validated and qualitative or semi-quantitative methods, separation in non-equilibrated conditions etc. Still, the TLC technique is popular because of its simplicity and fast results.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>HPTLC technique, features and advantages:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/anchrom.in\/hptlc\">HPTLC (High-Performance Thin Layer Chromatography)<\/a> is a modern version of TLC wherein the principle remains the same but the practice is fully automated and GLP\/USP\/EP and 21 CFR rule 11 compliant. HPTLC practice is standardized by United States Pharmacopoeia and the focus of HPTLC is on reproducibility and high separation power.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">A<strong>dvantages of HPTLC over TLC:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>HPTLC can be used for both qualitative and quantitative analysis of different samples e.g. Herbal, food, pharma, forensic, cosmetic, speciality chemicals etc.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>HPTLC is a high throughput technique, more than 100 samples can be run per day with minimal analysis cost.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>HPTLC enables multiple detections without repeating chromatography which means one can get many data points after doing a single analysis which gives thorough information about the sample applied.<\/li><\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Response of the analyte can be checked in UV, fluorescence and white light with a suitable TLC photo documentation unit. Chromatograms can be obtained with <a href=\"https:\/\/anchrom.in\/camag-pro-system\">CAMAG scanner 4 for quantitative analysis<\/a> and spectral evaluation. If required derivatization can be done with CAMAG derivatizer.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>HPTLC can also be coupled with a mass analyser for the identification of separated analytes by means of a TLC-MS interface.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>The results obtained are highly reproducible, reliable analytical results and come with traceable data and images.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>Highly standardized parameters are used for HPTLC analysis. HPTLC methods can be developed and validated as per suitable guidelines.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>HPTLC can also be coupled with biological detection methods e.g. antioxidant activity, antidiabetic activity, antimicrobial activity etc.<\/li><\/ul>\n\n\n\n<ul class=\"wp-block-list\"><li>&nbsp;HPTLC has applications in the herbal, food, pharma, forensic and cosmetic field.<\/li><\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">All the above features make HPTLC a unique, more advantageous and reliable analytical technique.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Anchrom Enterprises Pvt. Ltd is one of the leaders in&nbsp;HPTLC analysis.&nbsp;Please contact us at&nbsp;<\/em><\/strong><a href=\"mailto:lab@anchrom.in\"><strong><em>lab@anchrom.in<\/em><\/strong><\/a><strong><em>&nbsp;for HPTLC analysis of plant extracts, drugs, ingredients in cosmetics, and forensic science.<\/em><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Thin Layer Chromatography is a separation technique which is used to separate mixtures of organic compounds. This technique is in practice since 1960 and is been used for the separation of a lot of different compounds. <\/p>\n","protected":false},"author":4,"featured_media":782,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[4],"tags":[77,80,10,76,78,79],"class_list":["post-781","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hptlc-applications","tag-glp-usp-ep-compliant-chromatography","tag-high-performance-thin-layer-chromatography","tag-hptlc","tag-hptlc-applications","tag-tlc-21-cfr","tag-tlc-scanner-for-quantification"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>How is HPTLC different from TLC? | Anchrom<\/title>\n<meta name=\"description\" content=\"In this article, we have explained the features of the HPTLC technique and tried to differentiate HPTLC from TLC. 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