A novel approach for the development of the first universal HPTLC mixture (UHM) to be used in system suitability tests was developed by the research team at CAMAG Switzerland in collaboration with Anchrom Enterprises (I) Pvt Ltd, and Sigma Aldrich Chemie GmBH, Switzerland.
System Suitability Test:
Universal HPTLC Mix (UHM):
Chromatographic behavior of the UHM:
The chromatographic behavior of the UHM was evaluated using 20 different mobile phases on Silica Gel F254 and a baseline separation of at least 3 different constituents was done. Additionally, testing carried out in collaboration with four different labs showed the Rf value of the mobile phase was within the confidence interval of 0.040 Rf units.
The mixture was subjected to various conditions viz. changes in Relative Humidity, improper saturation, deliberate mistakes in the preparation of the mobile phase.
With reference to the various tests that were carried out for the qualification of the Universal HPTLC mixture, it can be concluded that the novel Universal HPTLC Mixture can be used for the System Suitability Testing.