High Performance Thin Layer
Chromatography (HPTLC) is chromatographic technique based on the principle of
planar chromatography. It is a modernized version of Thin Layer Chromatography
(TLC) with enhanced capabilities.
CAMAG is Swiss based company manufacturing
TLC since 1961. They are the world leaders in instrumental HPTLC. First Manual
TLC was into existence. Over the years the technique has evolved from manual to
semi-automatic which now has upgraded to fully automated HPTLC PRO system in
2019 for routine quality control.
HPTLC not only achieves miniaturization
of chromatograms but most importantly better resolution and reproducibility with a state of art software for documentation and
control over the instrumentation. It is cost
effective with high throughput. Up to 15 samples can be run on plate at a time
and with less solvent consumption.
CAMAG HPTLC has modules of instruments combined to form a system.
Each step has a designated module catering to analyst’s needs. These are
software- controlled instruments with 21 CFR PART 11 & GLP compliance.
Starting from applicator to detector and hyphenation. HPTLC produces visual
chromatograms and gives information unlike other techniques.
Chromatography is a technique for
separation of mixtures. HPTLC is one such powerful tool for separation of
mixtures. Apart from separating compounds/analytes of interest it can be useful
for various applications. Such as food testing, pharmaceuticals, forensics,
cosmetics, herbals, etc.
HPTLC’s use in chromatography is not just
limited to separation of mixtures. Various other applications in the field of
pharmaceuticals include identification and quantification of API can also be
performed using HPTLC. Vast applications like impurity profiling, content
uniformity test (CUT), validation studies, stability studies, degradation
studies and quantification.
Hence, HPTLC is an ideal choice for samples of various types with less
solvent consumption, accurate and fast results.
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